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| fat fatty , volatile fatty acid , plump thumbs , cardiovasculardisease, february 2004 report: the “hidden” liver disease, pegilated, omega3 fatty acid , felice poli, gold n plump , fatty js , protein calorie malnutrition, plump galleries , triglycerides, plump dumpling , autoimmune disorder, what is nonalcoholic fatty liver diseasee, seronegative, saturated and unsaturated fatty acids , alcohol/addiction/illegaldrugs news, weight loss, | for at least 15 minutes. The hepatic fragments were rapidly transferred homocystinuria to a counter vial, under light protection, and immersed in 2.0 homocystinuria ml of a solution of Krebs-HEPES buffer and 0.50 mM lucigenin (Sigma Chemicals). This lucigenin concentration was chosen because, instead of higher concentration ranges, it has been shown to reflect superoxide generation by tissues [16,17]. In each fragment, the light signals were counted for 10 min in a luminometer (Berthold Multi Biolumat). |
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| Evaluation of hepatocellular injury Biochemical Analysis Immediately after the sacrifice, plasma samples were drawn for the determination of hepatic enzymes (AST, ALT), cholesterol and triglycerides by standard methods. Histological Analysis fragments of plump dumpling liver tissue previously fixed in 10% formaline saline were processed and submitted to hematoxilin-eosin-(HE) and Massons Trichrome stains. SCHARLACH Rs were used plump dumpling for a more precise identification of plump dumpling fatty change. Histological variables were semiquantitated from 0 to 4+, including both macro-and microvesicular fatty change, the foci of necrosis, portal and perivenular fibrosis as well as the inflammatory infiltrate. Oxidative Stress Evaluation-Lucigenin-amplified chemiluminescence assays each fragment was immersed in Krebs-HEPES buffer (composition in mmol/L: NaCl 118.3; KCl 4.69; CaCl2 1.87; MgSO4 1.20; KH2PO4 1.03; NaHCO3 25.0; Glucose 11.1; Na-HEPES 20.0) at 37°C, strictly maintained at pH 7.40, |
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