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In addition, normal liver enzyme levels do not guarantee freedom from underlying steatohepatitis and advanced cirrhosis. There is thus a clinical dilemma of whether to subject the proteomic diagnostics patient to an invasive procedure, which carries some risk, in order to diagnose and stage a disease in apparently asymptomatic patients. A number of studies have addressed proteomic diagnostics clinical predictors of more advanced histology on the initial diagnostic biopsy (Table 1). Older age, increasing obesity, type 2 diabetes and hypertriglyceridaemia appear to be the strongest independent proteomic diagnostics predictors of more advanced disease (Angulo et al, 1999; Dixon et al, 2001). Other factors identified as predictors of advanced disease include female gender, impaired glucose tolerance, insulin resistance, high fasting C-peptide, hypertension, elevation of AST or ALT, and an AST:ALT ratio of >1.0. These factors are generally used to identify patients in whom liver biopsy may provide the most prognostic information.
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