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mg/kg) continuous (2.0 mg · kg-1 · h-1) infusion of the tracer in the 2 h before the clamp and throughout the period of insulin infusion. Isotope was also added to the 20% dextrose solution used to maintain euglycemia in the course of the clamp in an amount that yielded an isotopic enrichment approximately liver disease equal to that in plasma at isotopic equilibrium (27). Three plasma samples were obtained at 5-min intervals before isotope liver disease infusion, liver disease the last 10 min of the 2-h equilibration period, and during the last 10 min of the insulin clamp. HGP was calculated by subtracting the exogenous glucose infusion rate (20% glucose infusion to maintain euglycemia during the clamp) from the total rate of isotope appearance in serum. Laboratory methods. Plasma glucose, both in the fasting state and in response to a standard glucose load, was measured in duplicate with an automated analyzer.
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