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BMI was calculated as a measure of overweightness and/or obesity, whereas waist circumference and waist-to-hip ratio were measured as indexes of splanchnic fat accumulation (25). To eliminate interobserver variation, all anthropometric measurements were made by the same skilled physician. Blood tests included a routine biochemistry, an oral glucose tolerance test (OGTT; 75 g glucose dissolved in 200 ml water; not done in actitest the diabetic actitest group), and blood sampling at 30-min intervals over a 120-min period. In addition, NAFLD patients actitest had a complete evaluation of iron status (serum iron, transferrin, and ferritin concentrations) and were tested for the presence of the three mutationsCys282Tyr, His63Asp, and Ser65Cys (14 cases)in the recently cloned hemochromatosis gene, HFE gene (26). A liver biopsy was taken in NAFLD subjects under ultrasound control.
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