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All patients were screened for viral and autoimmune serum markers (hepatitis viruses A to G, cytomegalovirus, Ebstein-Barr, herpes simplex, anti-nuclear antibodies, anti-smooth muscle antibodies, anti-liver-kidney microsomal antibody, anti-DNA antibodies, anti-mitochondrial antibodies and perinuclear anti-neutrophil cytoplasmic antibodies). Tests for Wilson's disease and 1-antitrypsin deficiency were also done. Serum ferritin level had to be normal on two occasions at least one week apart. fat bottomed girls lyrics All medications received during this 24-week period were recorded. During the first visit, all patients underwent liver ultrasonography by two radiologists fat bottomed girls lyrics and upper abdominal CT scan. fat bottomed girls lyrics Obesity was defined as BMI >25.0.[15] Obese patients were advised weight reduction. Serum transaminases were monitored every 8 weeks during the 24-week surveillance period. At the end of the 24-week follow-up, ultrasonography was repeated by the same two radiologists and a percutaneous liver biopsies were performed without radiological guidance using Menghini needle (Hepafix; Braun, Germany) of 1.4
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